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KMID : 0613820070170050634
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Journal of Life Science
2007 Volume.17 No. 5 p.634 ~ p.640
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Effect of n-3 fatty acid deficiency on fatty acid compositions of nervous system in rats reared by artificial method
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Lim Sun-Young
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Abstract
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Our previous study suggested that n-3 fatty acid deficiency was associated with significantly reduced spatial learning as assessed by Morris water maze test. Here we investigated an effect of n-3 fatty acid deficiency on rat brain, retina and serum fatty acyl compositions at 15 wks age using a first generational artificial rearing technique. Newborn Rat pups were separated on day 2 and assigned to two artificial rearing groups or a dam-reared control group. Pups were hand fed artificial milk via custom-designed nursing bottles containing either 0.02% (n-3 Deficient) or 3.1% (n-3 Adequate) of total fatty acids as α-linolenic acid (LNA). At day 21, rats were weaned to either n-3 deficient or n-3 adequate pelleted diets and fatty acid compositions of brain, retina and liver were analyzed at 15 wks age. Brain docosahexaenoic acid (DHA) was lower (58% and 61%, P＜0.05) in n-3 deficient in comparison to n-3 adequate and dam-reared groups, receptively, while brain docosapentaenoic acid (DPAn-6) was increased in the n-3 deficient group. In retina and serum fatty acid compositions, the decreased precentage of DHA and increased precentage of DPAn-6 were observed. These results suggested that artificial rearing method can be used to produce n-3 fatty acid deficiency in the first generation and that adequate brain DHA levels are required for optimal brain function.
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KEYWORD
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Docosahexaenoic acid, artificial rearing system, brain function, n-3 fatty acid deficiency
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